Ho fumanoa ka ho Otloloha ha Likokoana-hloko tsa Likokoana-hloko

Letoto lena la mekhoa ke litlhahlobo ka ho sebelisa antigen e khethehileng ea kokoana-hloko ho bona li-antibodies ho serum ea bakuli, ho kenyeletsoa ho lemoha li-antibodies tsa IgM le tekanyo ea li-antibodies tsa IgG.Li-antibodies tsa IgM li nyamela ka mor'a libeke tse 'maloa, athe li-antibodies tsa IgG li ntse li tsoela pele ka lilemo tse ngata.Ho theha tlhahlobo ea ts'oaetso ea vaerase ho finyelloa ka serologically ka ho bonts'a ho phahama ha li-anti-titer ho kokoana-hloko kapa ka ho bonts'a li-antibodies tsa antiviral tsa sehlopha sa IgM.Mekhoa e sebelisoang e kenyelletsa tlhahlobo ea neutralization (Nt), complement fixation (CF) test, hemagglutination inhibition (HI) test, le immunofluorescence (IF) test, passive hemagglutination, le immunodiffusion.

A. Litlhahlobo tsa ho Neutralization

Nakong ea ts'oaetso kapa setso sa lisele, kokoana-hloko e ka thibeloa ke antibody ea eona e khethehileng 'me ea lahleheloa ke ts'oaetso, mofuta ona oa antibody o hlalosoa e le anti-antibody.Litlhahlobo tsa ho se nke lehlakore ke ho bona lesole la 'mele la ho se nke lehlakore ka har'a serum ea bakuli.

B. Tlatselletsa Litlhahlobo tsa ho Lokisa

Teko ea ho lokisa e tlatsanang e ka sebelisoa ho sheba boteng ba anti-antigen kapa antigen e itseng serum ea mokuli.Teko e sebelisa lisele tse khubelu tsa mali tsa linku (SRBC), anti-SRBC antibody le tlatsetso, mmoho le antigen e itseng (haeba e batla sethibela-mafu serum) kapa lesole la 'mele le itseng (haeba le batla antigen ho serum).

C. Hemagglutination Inhibition Assays

Haeba kokoana-hloko ea sampole e phahame, ha sampole e tsoakoa le li-RBCs, ho tla etsoa lesela la livaerase le li-RBC.Ketsahalo ena e bitsoa hemagglutination.Haeba li-antibodies khahlanong le hemagglutinins li le teng, hemagglutination e tla thibeloa.Nakong ea tlhahlobo ea hemagglutination inhibition, ho hlapolloa ka seriale ha serum ho kopanngoa le bongata bo tsejoang ba kokoana-hloko.Ka mor'a ho qhoqhoa, li-RBC li kenngoa, 'me motsoako o tloheloa hore o lule lihora tse' maloa.Haeba hemagglutination e thibetsoe, pellet ea RBCs e bopa ka tlase ho tube.Haeba hemagglutination e sa thibeloe, filimi e tšesaane e thehoa.