Ho tloha ka Pherekhong 2020 ho isa Mphalane 2020, boithuto bo lebelletsoeng ba mokhoa bo ile ba etsoa Sepetleleng sa Univesithi ea Pisa se phatlalalitsoeng ho BMC Microbiology.Goldstream®Fungus (1-3) -β-D-Glucan Test e ile ea sebelisoa bakeng sa ho lemoha boemo ba BDG ho tloha lisampole tsa BAL.Sephetho se ile sa lekanngoa ke aIGL-200 ka ho Fetisisa Kinetic Tube Readerho tsoa ho Era Biology.Patlisiso e bontša hore BDG e lokela ho nkeloa hloohong bakeng sa boleng ba eona bo phahameng bo bobe ba ho bolela esale pele.'Me ho ile ha fella ka thuso ea ho thibela ho hlahlojoa ha PCP bakeng sa bakuli bohle ba laolang hampe.
Ka morao:
Tekanyetso ea hajoale ea tlhahlobo ea khauta bakeng saPneumocystis jiroveciie emeloa ke pono e sa bonahaleng ea fungus ho tsoa lisampoleng tsa phefumoloho ea bongaka, joalo ka mokelikeli oa bronchoalveolar-lavage, o hlalosang "ho netefalitsoe"P. jiroveciipneumonia, athe qPCR e lumella ho hlalosa "mohlomong", kaha ha e khone ho khetholla tšoaetso ho tloha likolone.Leha ho le joalo, mekhoa ea limolek'hule, e kang end-point PCR le qPCR, e potlakile, e bonolo ho e etsa le ho e hlalosa, kahoo e lumella laboratori ho khutlisetsa ngaka lintlha tsa bohlokoa tsa microbiological ka nako e khutšoanyane.Boithuto ba hajoale bo ikemiselitse ho bapisa microscopy le liteko tsa limolek'hule le ts'ebetso ea tlhahlobo ea beta-D-glucan ho maro a bronchoalveolar-lavage ho tsoa ho bakuli ba belaelloang.Pneumocystis jiroveciinyomonea.Bronchoalveolar-lavage fluid ho tsoa ho tse leshome le metso e robeli tse kotsing e kholo le tse 'ne tsa taolo e mpe li kenetse Grocott-Gomori's methenamine silver-staining, end-point PCR, RT-PCR, le beta-D-glucan assay.
Liphetho:
Lisampole tsohle tse ntle tsa bronchoalveolar-lavage (50%) le tsona li hlahisitse PCR ea nako ea ho qetela le ea nako ea 'nete, empa tse peli, li bile le phello e ntle ka palo ea beta-D-glucan.Qetello ea PCR le RT-PCR e fumane 10 (55%) le 11 (61%) ho lisampole tsa 18, ka ho latellana, kahoo e bontša kutloisiso e ntlafetseng ha e bapisoa le microscopy.Kaofela RT-PCR e nang le Ct<27 e netefalitsoe ka microscopically, athe disampole tse nang le Ct≥ 27 li ne li se joalo.
Qetello:
Mosebetsi oa rona o totobatsa tlhoko ea ho hlophisa bocha le ho hlalosa bocha karolo ea tlhahlobo ea limolek'hule maemong a ikhethileng a kliniki, joalo kaP. jiroveciits'oaetso, e leng boemo bo sa tloaelehang empa hape bo matla le bo tsoelang pele ka potlako bo amang batho ba nang le immunocompromised ba ka ruang molemo haholo ho tsoa tlhahlobong e potlakileng.Bakuli ba khethiloeng ka thata, ho ea ka mekhoa ea ho kenyelletsa, ho ka ba le phello e mpe ka mekhoa ea limolek'huleP. jiroveciinyomonea.
Franconi I, Leonildi A, Erra G, et al.Papiso ea mekhoa e fapaneng ea microbiological bakeng sa tlhahlobo ea Pneumocystis jirovecii pneumonia ho bronchoalveolar-lavage fluid.BMC Microbiol.2022;22(1):143.E phatlalalitsoe 2022 May 21. doi:10.1186/s12866-022-02559-1
Nako ea poso: Jun-14-2022