Tlhahlobo ea K-Set ea IMP e hanyetsanang le Carbapenem (Lateral Flow Assay)
Kenyelletso ea Sehlahisoa
The Carbapenem-resistant IMP Detection K-Set (Lateral Flow Assay) ke mokhoa oa tlhahlobo oa immunochromatographic o reretsoeng ho lemoha boleng ba IMP-type carbapenemase likoloneng tsa baktheria.Teko ke tlhahlobo ea laboratori e sebelisoang ke ngaka e ka thusang ho hlahlobisisa mefuta ea IMP-mofuta oa carbapenem.
Litšobotsi
| Lebitso | Tlhahlobo ea K-Set ea IMP e hanyetsanang le Carbapenem (Lateral Flow Assay) |
| Mokhoa | Lateral Flow Assay |
| Mofuta oa mohlala | Likolone tsa baktheria |
| Tlhaloso | 25 liteko / kit |
| Nako ea ho lemoha | 10-15 mets |
| Lintho tsa ho lemoha | Enterobacteriaceae e hanyetsanang le Carbapenem (CRE) |
| Mofuta oa ho lemoha | IMP |
| Botsitso | K-Set e tsitsitse ka lilemo tse 2 ho 2°C-30°C |
Molemo
- Ka potlako
Fumana sephetho nakong ea metsotso e 15, matsatsi a 3 pele ho mekhoa ea khale ea ho lemoha - E bonolo
Ho bonolo ho e sebelisa, basebetsi ba tloaelehileng ba laboratori ba ka sebetsa ntle le koetliso - Nepahetse
Boikutlo bo phahameng le bo ikhethang
Tekanyo e tlase ea ho lemoha: 0.20 ng/mL
E khona ho bona boholo ba mefuta e menyenyane e tloaelehileng ea IMP
- Sephetho se hlakileng
Ha ho na tlhoko ea ho bala, sephetho sa ho bala ka pono - Moruo
Sehlahisoa se ka tsamaisoa le ho bolokoa mocheso oa kamore, ho fokotsa litšenyehelo
Bohlokoa ba tlhahlobo ea CRE
Ka kopanelo, Enterobacterales ke sehlopha se atileng haholo sa likokoana-hloko tse bakang mafu a amanang le tlhokomelo ea bophelo.Li-Enterobacterales tse ling li ka hlahisa enzyme e bitsoang carbapenemase e etsang hore lithibela-mafu tse kang carbapenems, penicillins le cephalosporins li se ke tsa sebetsa.Ka lebaka lena, CRE e ’nile ea bitsoa “libaktheria tse tšosang” hobane ho na le lithibela-mafu tse seng kae tse ling, haeba li teng, tse setseng ho phekola mafu a bakoang ke likokoana-hloko tsena.
Likokoana-hloko tse tsoang lelapeng la Enterobacterales, ho kenyeletsa le mofuta oa Klebsiella le Escherichia coli, li ka hlahisa carbapenemase.Hangata li-carbapenemase li hlahisoa ho tloha liphatsa tsa lefutso tse fumanehang holim'a lintho tse fetisetsoang tse ka jalang khanyetso habonolo ho tloha kokoana-hloko ho ea ho kokoana-hloko le motho ho ea ho motho.Hape ka lebaka la tšebeliso e mpe ea lithibela-mafu le mekhoa e fokolang e nkiloeng ho thibela ho ata, bothata bo ntseng bo eketseha ka sekhahla ba CRE bo fetoha tšokelo ea bophelo lefatšeng ka bophara.
Hangata, ho ata ha CRE ho ka laoloa ke:
- Ho beha leihlo mafu a CRE
- Arola bakuli ba nang le CRE
- Ho tlosa lisebelisoa tsa bongaka tse hlaselang ka hare ho 'mele
- E-ba hlokolosi ha u fana ka lithibela-mafu (haholo-holo carbapenems)
- Ho sebelisa mekhoa e hloekileng e hloekisitsoeng ho fokotsa ho ata ha tšoaetso
- Latela ka tieo kemiso ea ho hloekisa lab
……
Ho fumana CRE ho bohlokoa haholo taolong ea phatlalatso.Ka ho etsa liteko esale pele, bafani ba tsa bophelo bo botle ba ka fana ka kalafo e nepahetseng ho bakuli ba kotsing ea CRE, hape ba fihlela taolo ea sepetlele.
IMP-mofuta oa carbapenemase
Carbapenemase e bolela mofuta oa β-lactamase o ka khonang bonyane haholo hydrolyze imipenem kapa meropenem, ho akarelletsa le A, B, D mefuta e meraro ea li-enzyme tse khetholloang ke Ambler sebopeho sa molek'hule.Har'a bona, Sehlopha sa B ke metallo-β-lactamases (MBLs), ho akarelletsa le carbapenemase e kang IMP, VIM le NDM,.IMP-type carbapenemase, eo hape e tsejoang e le imipenemase metallo-beta-lactamase e hlahisang CRE, ke mofuta o atileng haholo oa li-MBL tse rekoang mme e tsoa ho subclass 3A.E ka etsa hydrolyze hoo e ka bang lithibela-mafu tsohle tsa β-lactam.
Tshebetso
- Kenya marotholi a 5 a tharollo ea sampole ea kalafo
- Kokobetsa likolone tsa baktheria ka loop ea ente e lahloang
- Kenya loop ka har'a tube
- Kenya 50 μL selibeng sa S, emela metsotso e 10-15
- Bala sephetho
Lintlha tsa Odara
| Mohlala | Tlhaloso | Khoutu ea sehlahisoa |
| CPI-01 | 25 liteko / kit | CPI-01 |
